nick translation

Apoptotic cells were specifically stained using in situ nick translation .

应用原位缺口平移法检测细胞凋亡。

Evaluation of radiosensitivity of nasopharyngeal cancer cells by in situ nick translation

原位缺口平移测定鼻咽癌细胞的放射敏感性

The Application of in Situ Nick Translation Labeling Technique on Apoptotic Cell in Rat

原位缺口平移法在检测睾丸组织凋亡细胞中的应用

The apoptosis of spermatogenic cells was studied with in situ nick translation ( ISNT ) technique .

采用原位缺口平移技术观察睾丸曲细精管中细胞凋亡数目的变化。

The a - ~ ( 32 ) p-labeled DNA probe was prepared by nick translation .

用缺口翻译法制备a-~（32）P标记探针。

In situ nick translation method reveals the antimutagenic effect of the total saponins of Cimicifuga dahurica

原位缺口翻译技术检测兴安升麻总皂甙的抗突变作用

Damage of DNA in hepatocytes were detected by in situ nick translation test after DEN treatment both in vivo and in primary cultured liver cells .

在体内和体外（无血清原代培养肝细胞）经DEN攻击后，以核酸原位缺口标记方法观察肝细胞DNA的损伤程度。

We evaluated the radiosensitivity of two nasopharyngeal cell lines which had been treated with γ - rays by in situ nick translation ( ISNT ) .

报道了以原位缺口平移（ISNT）技术研究鼻咽癌细胞株CNE、CNE－2Z对γ射线的放射效应，以及用存活曲线验证ISNT的可靠性。

Brain nuclei of rats exposed or unexposed to methylmercury chloride were digested by DNase 1 or EcoR 1 , and then nick translation was performed .

接触或不接触氯化甲基汞的大鼠脑细胞核分别经DNase1和EcoR1核酸内切酶消化后进行缺口翻译。

In situ nick translation labeling of DNA strand breaks : its application in differential detection of apoptosis and dying cells in the tissues from patients and experimen-tally infected animals with epidemic hemorrhagic fever

原位缺口平移标记断裂DNA技术及其在检测流行性出血热尸检和实验动物组织中细胞凋亡和早期死亡的应用

The [ α - ~ ( 32 ) P ] dATP of high specific activity prepared by enzymatic method is satisfactorily used for the labelling of DNA ( probe ) by nick translation and'molecular hybridization .

用该法制备的[α-~（32）P]dATP比活度高，能够满足遗传工程研究中进行DNA缺口翻译标记及分子杂交等工作的需要。

The whole HBV DNA sequences were labelled by random primer incorporation of Digoxigenin - labelled dUTP , and compared with ~ ( 32 ) P-labelled probe by nick translation .

按随机引物法以地高辛标记HBVdna探针，与按缺口翻译法以~（32）P标记的探针进行了比较。

The antimutagenic effect of the total saponins of Cimicifuga dahurica on mutagenicity of normal human peripheral blood cells induced by MMC were quantitatively detected by using in situ nick translation method and liquid scintillation counting .

本文采用原位缺口翻译技术，液闪计数测定，观察了兴安升麻总皂甙拮抗诱变剂丝裂霉素C（MMC）对人外周血细胞的致突变作用。

ENA of the virus was extracted by phenol-chloroform and precipitated by ethanol . cDNA probe was labeled by 33P according to nick translation method . Hybridization was carrying on under 42 ℃ .

采用酚-氯仿提取法和乙醇沉淀法提取标本中的RNA，cDNA探针以缺口翻译法标记~（32）P同位素，在42℃条件下进行cDNA-RNA分子杂交。

The methods of staining the testis cellular DNA and RNA with acridine orange , staining NOS with NADPH d histochemistry method , and observing the apoptosis number with in situ nick translation ( ISNT ) technique are used .

实验采用吖啶橙荧光染色法显示睾丸细胞DNA和RNA，黄递酶（NADPH-d）组织化学法显示睾丸细胞NOS，采用原位缺口平移技术检测睾丸生精细胞凋亡的数目。

DNA polymerase I mediated biotin dATP nick translation ( PANT ) and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling ( TUNEL ) were adopted for in situ identification of single and double strand DNA fragmentation , respectively .

采用原位PANT（DNA聚合酶I介导的生物素标记的dATP缺口平移）及原位TUNEL（末端脱氧核苷酸转移酶介导的dUTP末端标记），分别检测DNA损伤的单链断裂及双链断裂。